Products Descriptions
Intraclone BioPlus Yield is a chemical defined high-performance Cell Culture Supplement for Enhanced Titers & Product Quality at large scale production.
Intraclone BioPlus Yield is a revolutionary cell culture supplement specifically formulated to significantly boost fed-batch and perfusion culture productivity. It’s designed to deliver exceptionally high titers (6–10 g/L in fed-batch and 1–2 g/L/day in perfusion) while simultaneously improving product quality and reducing the cost of goods sold (COGS). The key to its success is its ability to control free radicals, which in turn reduces product heterogeneity—a persistent challenge in biomanufacturing. This results in superior monoclonal antibody (mAb) production with fewer aggregates, fragments, and other unwanted variants.
Overview
The Intraclone BioPlus Yield supplement is a cutting-edge solution engineered to address major bottlenecks in biomanufacturing. During high-density cell culture, particularly in fed-batch and perfusion systems, cellular metabolism can lead to the overproduction of reactive oxygen species (ROS), or free radicals. These highly reactive molecules cause oxidative stress, damaging cellular components and, critically, the therapeutic proteins being produced. This oxidative damage can manifest as product heterogeneity, a broad term encompassing various undesirable product variants, such as aggregation, fragmentation, oxidation, and the formation of low molecular weight species (e.g., H1L1). Such variations can compromise the efficacy and safety of the final therapeutic product.
Intraclone BioPlus Yield contains a proprietary blend of potent antioxidants and specialized nutrients that act as a powerful free radical scavenger. By neutralizing these harmful species within the bioreactor, the supplement mitigates oxidative stress and preserves the structural integrity of the expressed proteins. This not only enhances cell viability and productivity, leading to significant increases in volumetric productivity (titers) but also ensures a more homogenous product profile. The reduction in product heterogeneity simplifies downstream purification processes and reduces the risk of product loss, ultimately leading to a substantial decrease in the COGS. The supplement is easy to integrate into existing workflows and is suitable for a wide range of cell lines, including CHO, HEK293, NS0, and others..
How to Use It
To determine the optimal dilution of Intraclone Bioplus Yield for a specific cell line and process, a systematic Design of Experiments (DOE) approach is recommended. For more information, please refer to product Information brochure.
When to Add It: Add the reagent directly to your culture or during media preparation to boost cell growth
Need more information
write to us if you have any further questions, our Technical Services team is ready to assist.
For Research Use Only. Not for use in diagnostics procedures, Not human or Animal use
Applications
Intraclone BioPlus Yield is an indispensable tool for a variety of biomanufacturing applications:
- Fed-Batch Culture: Achieve titres of 6–10 g/L, maximizing production efficiency in a standard fed-batch process.
- Perfusion Culture: Drastically improve volumetric productivity by achieving titers of 1–2 g/L/day, enabling continuous manufacturing and high-throughput production.
- Monoclonal Antibody (mAb) Production: Specifically designed to address the challenges of mAb manufacturing, improving both yield and quality.
- Cell Line Development: Use in early-stage development to screen for optimal clones that produce high-quality protein.
- Process Optimization: Integrate into existing processes to improve performance and reduce the COGS without a major overhaul.
For Research Use Only. Not for use in diagnostics procedures, not human or Animal use
